People

RCNF

Jason T Kaelber, Ph.D.

Prof. Kaelber is the director of the RCNF since 2017. He has hands-on experience in all aspects of the cryoEM workflow, from cell culture to software development. He trained in cryoEM at the National Center for Macromolecular Imaging under Prof. Wah Chiu. Independent research in the Kaelber lab takes a structural biology approach to developing improved viruses for therapeutic applications and to understanding the origins of viral life. At the RCNF, Prof. Kaelber has facilitated discovery for researchers from biotechnology companies both large and small, as well as the pharmaceutical industry, diverse colleges & universities, federal research institutions, and many Rutgers investigators. He serves on the university’s Core Facility Advisory Committee.

Emre Firlar, Ph.D.

Dr. Firlar has over 10 years of experience with electron imaging of soft matter, including cells, bacteria, tissue, proteins, viruses, &c. During his graduate studies at Stevens Institute of Technology, post-doctoral work at Ames Laboratory, and post-doctoral work at the University of Illinois at Chicago, he drove projects using cryogenic, in situ liquid cell, and conventional electron microscopy including S/TEM and spatially-resolved EELS. He also has experience with Monte Carlo simulations of inelastic electron-matter interactions.

At the RCNF, Dr. Firlar has focused on single-particle cryoTEM, cryoET, cryoFIB lamella preparation, serial section imaging in FIB/SEM, and sample preparation.

Cryo-electron microscopists around Rutgers

The RCNF would like to call your attention to faculty members around the university with especial expertise in cutting-edge cryo-EM methods.

  • Prof. Lawson’s current projects involve structure database development (EMDataResourceNucleic Acid DatabaseProtein Data Bank), with emphasis on improving representation of large biological assemblies, including those determined using cryo-EM.
  • The Dai lab integrates cryo-electron tomography with CLEM and other tools to study nanomachines and disease-associated structures in their native cellular context, and has pioneered application of phase plates in biological electron microscopy.
  • The Kulczyk lab is developing new techniques in single-molecule CLEM as well as applying high-resolution single-particle analysis to biological targets.
  • The Petrou lab specializes in structural studies of transmembrane proteins using single-particle cryo-EM, with a particular focus on membrane enzymes linked to antibiotic resistance or membrane receptors linked to human physiology and pathology.

Our neighbors

We are housed on the ground floor of the Institute for Quantitative Biomedicine (Center for Integrative Proteomics Building) at 174 Frelinghuysen Rd, Piscataway, New Jersey on Rutgers’ Busch Campus.

Our facility is underneath the hard-working biocurators of the RCSB Protein Data Bank.

Also on our floor are two university core facilities, mass spectrometry and high-field NMR, and the super-resolution optical microscopy laboratory of Prof. Sanghyuk Lee.